Natural Food represent indispensablelity need its levying for the food of prawn larva. Although many plankton type able to be used as [by] prawn larva food, but for good natural food [of] hams fulfill some conditions, that is - Having size measure and form matching with prawn larva mouth - its Obstetrical [of] high him - solid Cell content and have flimsy cell wall, so that [is] easy to permeated - Quickly multiply and have tolerance which high enough to change of environmental factor - [Do] not [release] poisonous compound - Movement of it is not overactive, so that [is] easy to arrested by prawn larva.
To take care of the availibility of natural food for the larva of prawn, hence require to be [done/conducted] [by] natural food culture which with quality and optimal amount. Some plankton type which [is] often used for the food of prawn [is] Skeletonema-Costatum, Tetraselmis-Chilii, Chaetoceros-Calcitrans, Brachionus-Plicatilis and of Artemia-Sp.
TECHNIQUE CULTURE PLANKTON.
Pure Culture.
Way of this [done/conducted] in media [so that/ to be] to get one plankton species. Usually media [so that/ to be] fertilized with manure matching with wanted plankton. required equipments, for example, petridish, tail ose / mikropipet, erienmeyer, pipette, save microscope and reaction. Entire/All culture media and equipments except microscope, before used have to be sterilized in autoclave ( temperature 100°C, pressure 1 Atmosfir during 10 minute).
Condensation [so that/ to be] which have been fertilized, to be infused petridish, let a few moments become solidly. Take example [of] irrigate plankton with needle of ose / mikropipet and dab to surface of media [so that/ to be], [is] later;then closed and kept [at] room;chamber temperature. Few days later;then surface [so that/ to be] the have been growed [by] example [of] plankton colonys. By using microscope, each colony perceived and searched [by] plankton colony desired. If have been got, hence can be developed in tube react and used as [by] seed. If colony perceived still mixed with other type, hence the inokulasi have to repeat get pure colony. Pure colony [of] inkubasi in room which [is] ber-AC to take care of stability of temperature [among/between] 25 - 27°C.
Picture : 1. teknik kultur alami
source :
Ir. Sri Umiyati Sumeru
Dra. Suzy Anna
